Development of DNA aptamer selection approach based on membrane ultrafiltration of aptamer/target complex

Background. Aptamers are small single-stranded DNA or RNA molecules that have an affinity for a specific target molecule. The main method of aptamers construction is the technology systematic evolution ligands with exponential enrichment (SELEX). However, exact approach depends on nature molecules, and selected optimized by each researcher independently. article describes technique production to tick-borne encephalitis virus (TBEV) using membrane ultrafiltration molecular weight cut-off 100 kDa. As result, pool observable TBEV successfully enriched. aim. To develop suitable selection live, crude suspension directly in cell culture supernatant. Materials methods. was carried out modified SELEX aptamer combination semipermeable Vivaspin 6 (Sartorius, Germany) concentrators Enrichment performed real time polymerase chain reaction. were sequenced automated Sanger sequencing method. direct virucidal effect determined decrease titer infectious after incubation aptamer. Results. This expressed both antigen. Sixteen from this four them synthesized tested antiviral activity against TBEV. No observed. Conclusions. developed can be used select live viruses comparable size larger.